New insights into the treatment mechanisms of Vitamin D on PM2.5-induced toxicity and inflammation in mouse renal tubular epithelial cells

• DOI: 10.1016/j.intimp.2022.108747
• Publication Date: 2022-04-13T22:04:05Z
• Authors: Jing Huang; Peng Zhang; Qi An; Lan He; Lei Wang
• Keywords: Paricalcitol

Particulate matter (PM2.5) could induce renal injury other than lung and heart damage. The always displays in the way of inflammation. Vitamin D (VitD) has been identified as renal-protective factor with its anti-inflammatory effect.This study explored therapeutic effect VitD receptor (VDR) agonist (paricalcitol) on PM2.5-induced toxicity inflammation mouse tubular epithelial cells (mRTECs) molecular mechanisms.The variation between PM2.5 paricalcitol solution was investigated different concentration solutions transmission electron microscopy (TEM), laser-induced fluorescence (LIF) liquid chromatography-mass spectrometry (LC-MS). detoxication anti-inflammation effects vitro were analyzed by LIF, MTT, western blot (WB), ELISA flow cytometry (FCM).PM2.5 became more compact after treatment account stripped polycyclic aromatic hydrocarbons (PAHs). In cellular experiments, (160 μg/ml) evoked mRTECs apoptosis through NOD-, LRR- pyrin domain-containing protein 3 (NLRP3) /IL-1β axis, while (120 reversed these processes. intensity cell supernatant detected PAH-LIF weakened adding paricalcitol, compared with.PM2.5 alone. Interestingly, can significantly reduce highly toxic PAHs increase proportion nontoxic small PAHs. Furthermore, VDR expression negatively correlated apoptosis. summary, promote biological though PAH degradation from a effect, exert against NLRP3/IL-1β axis caused PM2.5.