Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalis

• DOI: 10.1016/j.isci.2019.10.032
• Publication Date: 2019-10-21T18:54:52Z
• Authors: Kumaran Sundaram; Daniel P. Miller; Anil Kumar; Yun Teng; Mohammed Sayed; Jingyao Mu; Chao Lei; Mukesh K. Sriwastva; Lifeng Zhang; Jun Yan; Michael L. Merchant; Liqing He; Yuan Fang; Shuangqin Zhang; Xiang Zhang; Juw W. Park; Richard J. Lamont; Huang-Ge Zhang
• Keywords: Exosome; Hemin; Pathogenic bacteria

Plant exosomes protect plants against infection; however, whether edible plant can mammalian hosts infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on surface of P. gingivalis. Compared PA (34:2), (34:1) did interact HBP35, indicating degree unsaturation plays critical role GELN-mediated interaction HBP35. On binding to pathogenic mechanisms were significantly reduced following cargo molecules, including and miRs. These molecules interacted multiple factors recipient bacteria simultaneously. Using as potential therapeutic agent prevent/treat chronic periodontitis was further demonstrated mouse model.