Novel Bacterial Diversity and Fragmented eDNA Identified in Hyperbiofilm-Forming Pseudomonas aeruginosa Rugose Small Colony Variant

• DOI: 10.1016/j.isci.2020.100827
• Publication Date: 2020-01-09T17:04:21Z
• Authors: Binbin Deng; Subhadip Ghatak; Subendu Sarkar; Kanhaiya Singh; Piya Das Ghatak; Shomita S. Mathew...; Sashwati Roy; Savita Khanna; Daniel J. Wozniak; David W. McComb; Chandan K. Sen
• Keywords: Extracellular polymeric substance

Pseudomonas aeruginosa biofilms represent a major threat to health care. Rugose small colony variants (RSCV) of P. aeruginosa, isolated from chronic infections, display hyperbiofilm phenotype. RSCV are highly resistant antibiotics and host defenses. This work shows that biofilm aggregates consist two distinct bacterial subpopulations uniquely organized displaying contrasting physiological characteristics. Compared with PAO1, the extracellular polymeric substance PAO1ΔwspF presented unique ultrastructural Unlike released fragmented DNA (eDNA) live cells. Fragmented eDNA, thus released, was responsible for resistance disruption by DNaseI. When added such eDNA enhanced formation. Disruption achieved aurine tricarboxylic acid, an inhibitor DNA-protein interaction. provides critical novel insights into structural functional characteristics hyperbiofilm-forming clinical variant relative its own wild-type strain.