Loss of the long non-coding RNA OIP5-AS1 exacerbates heart failure in a sex-specific manner

• DOI: 10.1016/j.isci.2021.102537
• Publication Date: 2021-05-13T16:27:48Z
• Authors: Aowen Zhuang; Anna C. Calkin; Shannen Lau; Helen Kiriazis; Daniel G. Donner; Yingying Liu; Simon T. Bond; Sarah C. Moody; Eleanor A.M. Gould; Timothy D. Colgan; Sergio Ruiz Carmona; Michael Inouye; Thomas Q. de Agui...; Elizabeth J. Tarling; Gregory A. Quaife...; James E. Hudson; Enzo R. Porrello; Paul Gregorevic; Xiao-Ming Gao; Xiao-Jun Du; Julie R. McMullen; Brian G. Drew
• Keywords: 0301 basic medicine; 570; Cardiovascular medicine; 0303 health sciences; Science; Q; 610; Article; 3. Good health; 03 medical and health sciences; Transcriptomics; Molecular physiology; Uncategorized

Long non-coding RNAs (lncRNAs) have been demonstrated to influence numerous biological processes, being strongly implicated in the maintenance and physiological function of various tissues including heart. The lncRNA OIP5-AS1 (1700020I14Rik/Cyrano) has studied several settings; however its role cardiac pathologies remains mostly uncharacterized. Using a series vitro ex vivo methods, we demonstrate that is regulated during development rodent human models disease settings mice. CRISPR, engineered global knockout (KO) mouse female KO mice develop exacerbated heart failure following pressure overload (transverse aortic constriction [TAC]) but male do not. RNA-sequencing wild-type hearts suggest regulates pathways impact mitochondrial function. Thus, these findings highlight as gene interest sex-specific differences failure.