TTF1 suppresses neuroblastoma growth and induces neuroblastoma differentiation by targeting TrkA and the miR-204/TrkB axis

• DOI: 10.1016/j.isci.2022.104655
• Publication Date: 2022-06-23T02:35:19Z
• Authors: Tianyou Yang; Jiahao Li; Zhenjian Zhuo; Huijuan Zeng; Tianbao Tan; Lei Miao; Manna Zheng; Jiliang Yang; Jing Pan; Chao Hu; Yan Zou; Jing He; Huimin Xia
• Keywords: Biological sciences; Cell biology; 03 medical and health sciences; 0302 clinical medicine; Oncology; Science; Q; Article; Cancer

Neuroblastoma (NB) is the most common extracranial malignant solid tumor in children. We found that TTF1, TrkA, and miR-204 were lowly expressed, whereas TrkB was highly expressed undifferentiated NB tissues. Meanwhile, TTF1 expression correlated positively with TrkA but negatively expression. The promoter hypermethylated tissues SK-N-BE cells, leading to downregulation. also identified miR-204, which directly targets TrkB, as a transcriptional target of TTF1. Functionally, suppressed proliferation, migration, invasion induced cell cycle arrest, apoptosis, autophagy cells by regulating miR-204-TrkB axis. Furthermore, growth promoted neurogenic differentiation xenograft mouse model. Our study demonstrates reduces induces targeting miR-204/TrkB