Sarco(endo)plasmic reticulum Ca2+-ATPase function is impaired in skeletal and cardiac muscles from young DBA/2J mdx mice

• DOI: 10.1016/j.isci.2022.104972
• Publication Date: 2022-08-18T03:57:02Z
• Authors: Riley E.G. Cleverdon; Jessica L. Braun; Mia S. Geromella; Kennedy C. Whitley; Daniel M. Marko; Sophie I. Hamstra; Brian D. Roy; Rebecca E.K. MacP...; Val A. Fajardo
• Keywords: SERCA; mdx mouse; Sarcolemma

The DBA/2J (D2) mdx mouse is a more severe model of Duchenne muscular dystrophy when compared to the traditional C57BL/10 (C57) mouse. Here, we questioned whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) function would differ in muscles from young D2 and C57 mice. Both mice exhibited signs impaired Ca2+ uptake gastrocnemius, diaphragm, left ventricle; however, level impairment was Reductions maximal SERCA activity were also prominent gastrocnemius diaphragm those mice; there no differences detected ventricle. Across all muscles, had highest levels oxidative stress as indicated by protein nitrosylation and/or nitration. In conclusion, our study shows that with age-matched