Single-molecule and super-resolved imaging deciphers membrane behavior of onco-immunogenic CCR5

• DOI: 10.1016/j.isci.2022.105675
• Publication Date: 2022-11-25T16:43:14Z
• Authors: Patrick Hunter; Alex L. Payne-Dwyer; Michael Shaw; Nathalie Signoret; Mark C. Leake
• Keywords: 0301 basic medicine; 03 medical and health sciences; Science; Immunology; Q; Biophysics; Article; Cancer; 3. Good health

The ability of tumors to establish a pro-tumorigenic microenvironment is an important point investigation in the search for new therapeutics. Tumors form microenvironments part by "education" immune cells attracted via chemotactic axes such as that CCR5-CCL5. Further, CCR5 upregulation cancer cells, coupled with its association features drug resistance and metastasis, has suggested therapeutic target. However, several conformational "pools" being reported, phenotypic investigations must be capable unveiling heterogeneity. Addressing this challenge, we performed super-resolution structured illumination microscopy (SIM) single molecule partially TIRF-coupled HILO (PaTCH) fixed cells. SIM data revealed non-random spatial distribution assemblies, while Intensity-tracking assemblies from PaTCH images indicated dimeric sub-units independent CCL5 perturbation. These biophysical methods can provide insights into structure function onco-immunogenic receptors many other biomolecules.