Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens

• DOI: 10.1016/j.isci.2023.105957
• Publication Date: 2023-01-13T10:05:38Z
• Authors: Ron Yefet; Nadav Friedel; Hadas Tamir; Ksenia Polonsky; Michael Mor; Lilach Cherry-Mimran; Eyal Taleb; David Hagin; Eli Sprecher; Tomer Israely; Natalia T. Freund
• Keywords: Monkeypox; Orthopoxvirus

Monkeypox virus (MPXV) resides in two forms; mature and enveloped, depending on it, distinct proteins are displayed the viral surface. Here, we expressed MPXV antigens from mature, one enveloped form, tested their reactivity to sera of 11 recoverees while comparing recently past vaccinated individuals. 8 out exhibited detectable neutralization levels against Vaccinia Lister. Sera all bound strongly A35R H3L antigens. Moreover, responses were significantly higher within compared both donors. Lastly, A35R- H3L-specific IgG+ B cells ranging 0.03-0.46% 0.11–0.36%, respectively, detected (A35R), 9 (H3L). Therefore, represent immune targets could be used a heat-inactivated serological ELISA for identification recent infection.