Distinct but interchangeable subpopulations of colorectal cancer cells with different growth fates and drug sensitivity

• DOI: 10.1016/j.isci.2023.105962
• Publication Date: 2023-01-13T10:05:41Z
• Authors: Roberto Coppo; Jumpei Kondo; Keita Iida; Mariko Okada; Kunishige Onuma; Yoshihisa Tanaka; Mayumi Kamada; Masayuki Ohue; Kenji Kawada; Kazutaka Obama; Masahiro Inoue
• Keywords: Spheroid; Clonogenic assay; Organoid

Dynamic changes in cell properties lead to intratumor heterogeneity; however, the mechanisms of nongenetic cellular plasticity remain elusive. When fate each from colorectal cancer organoids was tracked through a clonogenic growth assay, cells showed wide range ability even within clonal organoids, consisting distinct subpopulations; generating large spheroids and small spheroids. The generated only (S-pattern), while both (D-pattern), which were tumorigenic. Transition S-pattern D-pattern occurred by various extrinsic triggers, Notch signaling Musashi-1 played key role. resistant chemotherapy transited upon drug treatment signaling. As transition is linked resistance, it can be therapeutic target.