Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise

• DOI: 10.1016/j.isci.2023.106532
• Publication Date: 2023-03-31T07:58:45Z
• Authors: Yanli Yu; Xiaolu Zhang; Yanjing Chen; Yanze Li; Suying Bian; Yizhuo Yang; Hanan Song; Cheng Zhang; Zhenhe Dong; Guanghui Li; Luyou Xu; Zhuang Jia; Yu Liu; Xiaoyi He; Ming Yang; Jianjun Guo; Zhixiong Zhou; Yan Zhang
• Keywords: Monocyte; Exercise intensity; Intensity; Physical exercise

Vigorous-intensity leisure-time physical activity, such as marathon, has become increasingly popular, but its effect on immune functions and health is poorly understood. Here, we performed scRNA-seq analysis of peripheral blood mononuclear cells (PBMCs) after a bout symptom-limited cardiopulmonary exercise (CPX) test or marathon. Time-series single-cell revealed the detailed series landscapes in response to short long vigorous-intensity activities. Reduction effective T was observed with cell migration motility pathways enriched circulation following Baseline values PBMCs abundance were reached around 1 h CPX 24 longer time required for expression recovery cytotoxicity genes. The ratio effector/naive found change uniformly among participants could serve better indicator intensity than CD4+/CD8+ ratio. Moreover, identified time-dependent monocyte state transitions