Adipose tissue plasticity in pheochromocytoma patients suggests a role of the splicing machinery in human adipose browning

• DOI: 10.1016/j.isci.2023.106847
• Publication Date: 2023-05-09T21:57:02Z
• Authors: Moisés Castellá; Albert Blasco-Roset; Marion Peyrou; Aleix Gavaldà-Nav...; Joan Villarroya; Tania Quesada-López; Leyre Lorente-Poch; Juan Sancho; Florian Szymczak; Anthony Piron; Sonia Rodríguez-F...; Stefania Carobbio; Albert Goday; Pere Domingo; Antonio Vidal-Puig; Marta Giralt; Décio L. Eizirik; Francesc Villarroya; Rubén Cereijo
• Keywords: Biopsy sample, Specialized functions of cells, Transcriptomics; Biopsy; Science; Q; Specialized functions of cells; Généralités; Adipose tissues; Pheochromocytoma; Article; Teixit adipós; Feocromocitoma; RNA; Mineria de dades; Biopsy sample; Biòpsia; Transcriptomics; Data mining

Adipose tissue from pheochromocytoma patients acquires brown fat features, making it a valuable model for studying the mechanisms that control thermogenic adipose plasticity in humans. Transcriptomic analyses revealed massive downregulation of splicing machinery components and regulatory factors browned patients, with upregulation few genes encoding RNA-binding proteins potentially involved regulation. These changes were also observed cell culture models human adipocyte differentiation, confirming potential involvement cell-autonomous browning. The coordinated are associated profound modification expression levels splicing-driven transcript isoforms specialized metabolism adipocytes those master transcriptional regulators Splicing appears to be relevant component gene allow acquire phenotype.