Plasmon resonance biosensor for interleukin-1β point-of-care determination: A tool for early periodontitis diagnosis

• DOI: 10.1016/j.isci.2023.108741
• Publication Date: 2023-12-14T22:44:52Z
• Authors: Nunzio Cennamo; Debora Bencivenga; Marco Annunziata; Francesco Arcadio; Emanuela Stampone; Angelantonio Picc...; Fulvio Della Ragione; Luigi Zeni; Luigi Guida; Adriana Borriello
• Keywords: Point of care

Among pro-inflammatory cytokines, Interleukin-1β is crucially involved in several inflammatory-based diseases and even cancer. Increased levels oral fluids have been proposed as an early marker of periodontitis, a broadly diffused chronic inflammatory condition periodontal-supporting tissues, leading eventually to tooth loss. We describe the development portable surface-plasmon-resonance-based optical fiber probe suitably coated with anti-Interleukin-1β antibody monolayer. A pico-nanomolar linear range determination was obtained both buffer solution saliva rapid (3 min) incubation high selectivity presence interferents. Higher concentration periodontitis patient compared healthy control determined. These measurements were validated by automated ELISA system. Our results sustain potential applicability SPR-POF diagnostic point-of-care device for real-time monitoring salivary Interleukin-1β, that can support detection pathologies timely therapeutic decisions.