An intra-oral flavor detection task in freely moving mice

• DOI: 10.1016/j.isci.2024.108924
• Publication Date: 2024-01-16T07:49:35Z
• Authors: Kazuki Shiotani; Yuta Tanisumi; Yuma Osako; Koshi Murata; Junya Hirokawa; Yoshio Sakurai; Hiroyuki Manabe
• Keywords: Biological sciences; 0303 health sciences; 03 medical and health sciences; Behavioral neuroscience; Physiology; Science; Natural sciences; Q; Sensory neuroscience; Article; Neuroscience

Flavor plays a critical role in the pleasure of food. Flavor research has mainly focused on human subjects and revealed that many brain regions are involved in flavor perception. However, animal models for elucidating the mechanisms of neural circuits are lacking. Herein, we demonstrate the use of a novel behavioral task in which mice are capable of flavor detection. When the olfactory pathways of the mice were blocked, they could not perform the task. However, behavioral accuracy was not affected when the gustatory pathway was blocked by benzocaine. These results indicate that the mice performed this detection task mainly based on the olfaction. We conclude that this novel task can contribute to research on the neural mechanisms of flavor perception.