Protein kinase D drives the secretion of invasion mediators in triple-negative breast cancer cell lines

• DOI: 10.1016/j.isci.2024.108958
• Publication Date: 2024-01-17T02:04:10Z
• Authors: Alexia Gali; Irene V. Bijnsdorp; Sander R. Piersma; Thang V. Pham; Elena Gutiérrez-G...; Fiona Kühnel; Nikos Tsolakos; Connie R. Jimenez; Angelika Hausser; Leonidas G. Alexo...
• Keywords: Triple-negative breast cancer; Cancer cell lines

The protein kinase D (PKD) family members regulate the fission of cargo vesicles at Golgi complex and play a pro-oncogenic role in triple-negative breast cancer (TNBC). Whether PKD facilitates secretion tumor-promoting factors TNBC, however, is still unknown. Using pharmacological inhibition activity siRNA-mediated depletion PKD2 PKD3, we identified PKD-dependent secretome TNBC cell lines MDA-MB-231 MDA-MB-468. Mass spectrometry-based proteomics antibody-based assays revealed significant downregulation extracellular matrix related proteins pro-invasive such as LIF, MMP-1, MMP-13, IL-11, M-CSF GM-CSF PKD-perturbed cells. Notably, these cells was predominantly controlled by enhanced spheroid invasion. Consistently, more pronounced metastatic lines. Our study thus uncovers novel releasing secretome.