HIV-1 env gene mutations outside the targeting probe affects IPDA efficiency

• DOI: 10.1016/j.isci.2024.109941
• Publication Date: 2024-05-08T02:31:23Z
• Authors: Zehua Zhou; Xinyu Zhang; Meiyu Wang; Fei Jiang; Jincheng Tong; Jianhui Nie; Chenyan Zhao; Haifa Zheng; Zhen Zhang; Penghui Shi; Weiguang Fan; Youchun Wang; Weijin Huang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Virology; Science; Methodology in biological sciences; Q; Article

The intact proviral DNA assay (IPDA) based on droplet digital PCR was developed to identify and quantify HIV-1 latency reservoirs in patients infected with HIV-1. However, the genetic characteristics of different subtypes are non-consistent due their high mutation recombination rates. Here, we identified that IPDA sequences features an subtype could not effectively detect diversity Furthermore, demonstrated mutations