Rapid diffused optical imaging for accurate 3D estimation of subcutaneous tissue features

• DOI: 10.1016/j.isci.2025.111818
• Publication Date: 2025-01-23T01:09:36Z
• Authors: Shanshan Cai; John Mai; Winn Hong; Scott E. Fraser; Francesco Cutrale
• Keywords: optical imaging; biophysics; Science; cell biology; Q

Conventional light imaging in living tissues is limited to depths under 100 μm by the significant tissue scattering. Consequently, few commercial devices can image lesions beneath surface, or measure their invasion depth, critical dermatology. We present 3D-multisite diffused optical (3D-mDOI) an approach that combines photon migration techniques from diffuse tomography, with automated controls and analysis for estimating lesion's depth via its coefficients. 3D-mDOI a non-invasive, low-cost, fast, contact-free instrument capable of subcutaneous structures volumes through multisite-acquisition re-emitted diffusion on sample surface. It offers rapid estimation Breslow essential staging melanoma. To standardize performance, employs customized calibrations using physical phantoms, explore system's 3D reconstruction capabilities. find reconstruct up 5 mm below requiring ∼300 s computation time.