Transcriptome analysis to elucidate hexanal's mode of action in preserving the post-harvest shelf life and quality of banana fruits (Musa acuminata)

• DOI: 10.1016/j.jafr.2021.100114
• Publication Date: 2021-01-23T09:44:38Z
• Authors: Peninah Yumbya; Jane Ambuko; Margaret Hutchinson; Willis Owino; John Juma; Eunice Machuka; J. Musembi Mutuku
• Keywords: Expansin; Climacteric

Banana is a climacteric fruit whose ripening once initiated irreversible and proceeds very fast making the fruits highly perishable. Application of various post-harvest technologies has been shown to slow down process in such as banana. One these hexanal, naturally occurring compound that delays banana without compromising quality. As molecular mechanisms underlying mode action hexanal delaying are yet be elucidated, we undertook comparative transcriptomic analysis using treated with either ethylene or untreated controls. Results our study show significantly delayed rate pulp softening throughout storage period. Sequencing results showed 776 genes were up-regulated 2146 down-regulated upon treatment while 4 76 at day one storage. Additionally, 2423 2862 total 4820 5395 four. We found transiently suppressed expression involved biosynthesis, cell membrane deterioration wall degradation by four contrary observed induction same ethylene-treated fruits. The particular are; Aminocyclopropane-1-Carboxylic Acid Oxidase, 1-Aminocyclopropane-1-Carboxylic Acid, Phospholipase D, Polygalacturonase, Expansin Xyloglucan Endotransglucosylase. Later on 18 storage, aroma synthesis induced hexanal-treated These findings reveal works temporarily suppressing deterioration.