[P1–211]: MICRORNA‐140 MEDIATED REGULATION OF ADAM10

• DOI: 10.1016/j.jalz.2017.06.031
• Publication Date: 2017-10-20T08:45:42Z
• Authors: Rumana Akhter; Yvonne Shao; McKenzie Shaw; Shane Formica; Maria Khrestian; James B. Leverenz; Lynn M. Bekris
• Keywords: ADAM10

Recent reports in Alzheimer's Disease (AD) research suggests that alterations microRNA (miRNA) expression is associated with disease pathology. Our previous studies show genetic variation ADAM10 (A Disintegrin and Metalloproteinase 10) domain which encodes the major α-secretase responsible for cleaving Amyloid Precursor Protein (APP) with: higher CSF sAPP α levels cognitively normal controls compared AD patients along protein subjects low plaque scores those high scores, promoter activity promoter-only reporter constructs 3’ untranslated region (3’UTR) human neuroblastoma SHSY5Y cell line. These findings suggest ADAM10expression modulated according to an extended regulatory includes 3’UTR. In this study we have investigated role of trans-acting factors; transcription factor SOX2 miRNA-140, gene regulation. The entire sequence analyzed by computational bioinformatics screened probable trans-actors importance. shows miRNA-140–5p has enhanced post-mortem brain hippocampus using throughput micro-arrays qRT-PCR. Interestingly also seen miRNA-140 seed present on both 3’UTR thus containing elements were transfected into lines mimics inhibitors evaluate their interaction dual luciferase assays. specific its signifies importance miRNA controlling expression. Thus our proposed investigation unravels mechanisms underlying down-regulation miR-140 exacerbates related neurotoxic effects. could reflect a strong basis future aimed at understanding potential contribution factors as biomarker or modulator pathophysiology.