Quantitative proteomics reveals distinct composition of amyloid plaques in Alzheimer's disease

• DOI: 10.1016/j.jalz.2018.10.006
• Publication Date: 2019-01-02T13:33:26Z
• Authors: Feng Xiong; Wei Ge; Chao Ma
• Keywords: Laser capture microdissection; Amyloid (mycology)

Abstract Introduction We investigated the proteomic profiles of amyloid plaques (APs) from Alzheimer's disease (AD) and age‐matched non‐AD brains APP/PS1 transgenic model mice. Methods APs adjacent control regions were collected fresh‐frozen brain sections using laser capture dissection. Proteins quantitated tag‐labeling coupled high‐throughput mass spectra. Results Over 4000 proteins accurately quantified, more than 40 identified as highly enriched in both AD APs, including apoE, midkine, VGFR1, complement C4. Intriguingly, synaptic structural C1r, C5, C9 found to be upregulated but not APs. Moreover, pattern was distinct exhibited correlation with aging hippocampus. Discussion Our results provide new insight into AP composition. demonstrate unexpected differences between AD, non‐AD, mouse which may relate different pathological processes.