A novel method for silkworm cocoons self-degumming and its effect on silk fibers

• DOI: 10.1016/j.jare.2022.12.005
• Publication Date: 2022-12-23T16:43:46Z
• Authors: Riyuan Wang; Yuancheng Wang; Jianxin Song; Chi Tian; Xinyuan Jing; Ping Zhao; Qingyou Xia
• Keywords: Sericin; Fibroin

Conventional hot-alkaline cocoon degumming techniques greatly weaken the physicochemical and mechanical properties of silk fibroin fiber, thus affecting quality fabric. Moreover, it causes massive energy waste serious environmental pollution.This study aims to establish a novel self-degumming method by genetic modification silkworm varieties fibers.The self-degummed material was generated specifically overexpressing trypsinogen protein in sericin layer thread; effect evaluated rate protein, cleanliness equivalent diameter fiber; basic characteristics fiber degummed conventional technique were determined electron microscopy, Fourier infrared spectroscopy, X-ray diffraction tensile tests; composition biological activity respectively analyzed liquid chromatograph-mass spectrometry cytological experiments.The genetically engineered containing successfully created, content 47.14 ± 0.90 mg/g. The removed out water or 1 mM Tris-HCl buffer (pH = 8.0). Compared alkaline-degummed fibroin, had better cleanliness, thicker diameter, more complete structure property. In addition, from cocoons significantly promoted cell proliferation caused no obvious cytotoxicity.Compared technique, thread can mild condition, gain with biologically active products. This strategy not only reduce impact, but also generate greater economic value, which will accelerate its application pharmaceutical industries.