Glucagon changes substrate preference in gluconeogenesis

• DOI: 10.1016/j.jbc.2022.102708
• Publication Date: 2022-11-17T03:42:23Z
• Authors: Huiting Xu; Yujue Wang; Hyokjoon Kwon; Ankit Shah; Katarzyna Kalemba; Xiaoyang Su; Ling He; Fredric E. Wondis...
• Keywords: Gluconeogenesis; Carbohydrate Metabolism; Pyruvic acid

Fasting hyperglycemia in diabetes mellitus is caused by unregulated glucagon secretion that activates gluconeogenesis (GNG) and increases the use of pyruvate, lactate, amino acids, glycerol. Studies GNG hepatocytes, however, tend to test a limited number substrates at nonphysiologic concentrations. Therefore, we treated cultured primary hepatocytes with three identical substrate mixtures pyruvate/lactate, glutamine, glycerol serum fasting concentrations, where different U-