Comprehensive characterization of complex glycosphingolipids in human pancreatic cancer tissues

• DOI: 10.1016/j.jbc.2023.102923
• Publication Date: 2023-01-19T20:20:26Z
• Authors: Karel Hořejší; Chunsheng Jin; Zuzana Vaňková; Robert Jirásko; Ondřej Strouhal; Bohuslav Melichar; Susann Teneberg; Michal Holčapek
• Keywords: Characterization

Pancreatic ductal adenocarcinoma (PDAC) is one of the most common causes cancer-related deaths worldwide, accounting for 90% primary pancreatic tumors with an average 5-year survival rate less than 10%. PDAC exhibits aggressive biology, which, together late detection, results in patients presenting unresectable, locally advanced, or metastatic disease. In-depth lipid profiling and screening potential biomarkers currently appear to be a promising approach early detection other cancers. Here, we isolated characterized complex glycosphingolipids (GSL) from normal tumor tissues using combination TLC, chemical staining, carbohydrate-recognized ligand-binding assay, LC/ESI-MS2. The major neutral GSL identified were terminal blood groups A, B, H, Lea, Leb, Lex, Ley, P1, PX2 determinants globo- (Gb3 Gb4) neolacto-series (nLc4 nLc6). We also revealed that profiles their relative amounts differ between tissues. Additionally, type 1/2 core chains. 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