Functionalized nanogel for treating activated astrocytes in spinal cord injury

• DOI: 10.1016/j.jconrel.2020.12.006
• Publication Date: 2020-12-10T10:06:56Z
• Authors: Simonetta Papa; Valeria Veneruso; Emanuele Mauri; Giada Cremonesi; Xhuljana Mingaj; Alessandro Mariani; Massimiliano De P...; Arianna Rossetti; Alessandro Sacchetti; Filippo Rossi; Gianluigi Forloni; Pietro Veglianese
• Keywords: Nanogel; Astrogliosis; Internalization; Pinocytosis

Astrogliosis has a unique reaction during spinal cord damage, with helpful or adverse impacts on recovery. There is consequently a pressing need for treatment to target activated astrocytes and their unsafe response after injury to ensure some preservative effect during the progressive damage. We specifically developed and characterized a functionalized nanogel-based nanovector in vitro and in vivo, demonstrating its selectivity towards astrocytes, and limited uptake by macrophages when functionalized with both NH2 and Cy5 groups. In vitro experiments showed that the internalization was mediated by a clathrin-dependent endocytic pathway. After internalization into the cytoplasm of astrocytes, nanogels undergo lysosomal degradation and release compounds with potential therapeutic efficacy.