A cell-free, biomimetic hydrogel based on probiotic membrane vesicles ameliorates wound healing

• DOI: 10.1016/j.jconrel.2023.12.011
• Publication Date: 2023-12-19T23:50:46Z
• Authors: Thomas Kuhn; Ahmad Aljohmani; Nicolas Frank; Lina Zielke; Mina Mehanny; Matthias W. Laschke; Marcus Koch; Jessica Hoppstädter; Alexandra K. Kiemer; Daniela Yildiz; Gregor Fuhrmann
• Keywords: Lactobacillus casei

Probiotic bacteria, such as Lactobacilli, have been shown to elicit beneficial effects in various tissue regeneration applications. However, their formulation living bacteria is challenging, and therapeutic use proliferating microorganisms especially limited immunocompromised patients. Here, we propose a new avenue circumvent these shortcomings by developing bacteriomimetic hydrogel based on membrane vesicles (MVs) produced Lactobacilli. We coupled MVs from Lactobacillus plantarum casei, respectively, the surface of synthetic microparticles, embedded those bacteriomimetics into pharmaceutically applicable matrix. The wound microenvironment changes during healing process, including adaptions pH oxygen supply. thus performed proteomic characterization harvested under different culture conditions identified characteristic proteins related biological effect probiotics every state. In addition, highlight number unique expressed sorted for condition. Using vitro models, demonstrated that increased cell migration anti-inflammatory microparticles were dependent condition secreting bacteria. Finally, hydrogel's ability improve an vivo mouse full-thickness model. Our results create solid basis future application probiotic-derived treatment inflammatory dispositions stimulates initiation further preclinical trials.