Cellulose-degrading bacteria improve conversion efficiency in the co-digestion of dairy and chicken manure by black soldier fly larvae

• DOI: 10.1016/j.jenvman.2023.119156
• Publication Date: 2023-10-12T10:52:00Z
• Authors: Jia Zhang; Zhijun Luo; Nan Li; Yongqiang Yu; Minmin Cai; Longyu Zheng; Fengling Zhu; Feng Huang; Jeffery K.Tomberlin; Kashif ur Rehman; Ziniu Yu; Jibin Zhang
• Keywords: 2. Zero hunger; Manure; Larva; Diptera; Animals; Cellulases; Digestion; Cellulose; Chickens; Bacillus subtilis

Black soldier fly larvae (BSFL) have potential utility in converting livestock manure into larval biomass as a protein source for feed. However, BSFL limited ability to convert dairy (DM) rich lignocellulose. Our previous research demonstrated that feeding with mixtures of 40% and 60% chicken (DM40) provides novel strategy significantly improving their efficiency DM. the mechanisms underlying efficient conversion DM40 by are unclear. In this study, we conducted holistic study on taxonomic stucture functions microbiota gut during DM BSFL, well effects cellulosic biodegradation production. Results showed can consume cellulose other nutrients more effectively harvest shorter cycle system. The system yielded higher complexity. Bacillus Amphibacillus were strongly correlated degradation capacity. Furthermore, vitro screening results culturable cellulolytic microbes from guts isolated microbes. A key bacterial strain (DM40L-LB110; subtilis) high cellulase activity was validated industrial applications. Therefore, mixing an appropriate proportion increased abundance intestinal bacteria (Bacillus Amphibacillus) producing improved digestion (particularly degradation) cellulose-rich through changes microbial communities composition intestine. This reveals microecological high-efficiency provide applications large-scale wastes combined BSFL.