Botanical formulation, TADIOS, alleviates lipopolysaccharide (LPS)-Induced acute lung injury in mice via modulation of the Nrf2-HO-1 signaling pathway

• DOI: 10.1016/j.jep.2021.113795
• Publication Date: 2021-01-08T23:42:35Z
• Authors: Wonwoo Lee; Chang Hyung Lee; Jungkyu Lee; Yoonseon Jeong; Jong-Hyung Park; In-Jeong Nam; Doo Suk Lee; Hyun Myung Lee; Jaehyun Lee; Nayoung Yun; Jisun Song; Sooyeon Choi; Sunyoung Kim
• Keywords: Inflammation; Lipopolysaccharides; Male; 0301 basic medicine; NF-E2-Related Factor 2; Plant Extracts; Acute Lung Injury; Anti-Inflammatory Agents; Membrane Proteins; Medicine, Korean Traditional; Article; Antioxidants; Up-Regulation; 3. Good health; Mice, Inbred C57BL; Mice; Oxidative Stress; 03 medical and health sciences; RAW 264.7 Cells; Animals; Cytokines; Reactive Oxygen Species; Heme Oxygenase-1; Signal Transduction

TADIOS is an herbal formulation prepared from a mixture of Taraxacum officinale (L.) Weber ex F.H.Wigg, Dioscorea batatas Decaisne and Schizonepeta tenuifolia (Benth.) Briquet. These plants have traditionally been used in Asia to treat variety respiratory diseases. A bulk literature on traditional Korean medicine describe their activities functions for problems. Therefore, we hypothesized that the combination these might be effective alleviating symptoms.In this study, investigated whether ameliorates LPS-induced acute lung injury via regulation Nrf2-HO-1 signaling pathway.The mouse model was determine anti-inflammatory anti-oxidative stress effects TADIOS. The amount marker compounds contained quantified using high-performance liquid chromatography (HPLC) analysis. protein level pro-inflammatory cytokines culture supernatant measured by ELISA. Changes RNA mice lungs RAW264.7 cells were quantitative RT-PCR. relative amounts reactive oxygen species (ROS) DCF-DA assay. Western blot analysis evaluate expression cellular proteins. Effects antioxidant responsive elements (AREs) determined luciferase severity evaluated Hematoxylin & Eosin (H&E) staining. To test oxidative stress, myeloperoxidase (MPO) activity total capacity measured.TADIOS extraction blend three ethanol, quality control performed through quantification HPLC measurement bioactivities cell-based bioassays. In murine macrophage cell line RAW264.7, effectively suppressed production such as IL-6 IL-1β, also ROS induced LPS. When transfected with reporter plasmid containing nucleotide sequences AREs, treatment increased units dose-dependent manner. model, orally administered alleviated damage neutrophil infiltration Consistent vitro data, inhibited LPS-mediated activated axis.Our data suggest potential developed safe therapeutics distress syndrome.