Supplementation of dietary heptadecanoic acid enhances anti-Listeria monocytogenes response in macrophages

• DOI: 10.1016/j.jff.2024.106359
• Publication Date: 2024-07-17T03:06:18Z
• Authors: Qian Lei; Yaoyu Zhang; Tongtong Yang; Lei Chen; Xinxin Pei; Yujie Zhang; Tengfei Ma; Yuzhu Xie; Yi Wang; Hongxia Li; Cheng Cao; Buchang Zhang; Changzhi Xu
• Keywords: 0303 health sciences; 03 medical and health sciences; Nutrition. Foods and food supply; Macrophages; TX341-641; Heptadecanoic acid; Listeria monocytogenes; Sheep tail fat

Listeria monocytogenes infection often leads to listeriosis in immunocompromised individuals, and screening for potential nutrients that improve immunity may be beneficial. Here, we found that pre-incubation with heptadecanoic acid (C17:0), an odd-chain saturated fatty acid, significantly inhibited L.monocytogenes replication in differentiated THP-1 and RAW264.7, as evidenced by comparison of listeriolysin expression and colony count. Mechanistically, the administration of C17:0 triggered the activation of the IFN and NF-κB signaling pathways. Furthermore, C17:0 upregulated ACOD1 expression and the generation of itaconate, an anti-bacterial metabolite in macrophages. Based on these results, our comparative analysis showed that sheep tail fat (STF), ruminant lipid rich in C17:0, had similar anti-L.monocytogenes effect. Collectively, our data highlighted the function of C17:0 in boosting the immune response, shedding light on the application of C17:0 and STF as potential candidates for strengthening anti-L.monocytogenes response.