AKT-targeted anti-inflammatory activity of Panax ginseng calyx ethanolic extract

• DOI: 10.1016/j.jgr.2017.06.003
• Publication Date: 2017-06-24T01:16:25Z
• Authors: Sang Yun Han; Juewon Kim; Eunji Kim; Su Hwan Kim; Dae Bang Seo; Jong-Hoon Kim; Song Seok Shin; Jae Youl Cho
• Keywords: Calyx

Korean ginseng (Panax ginseng) plays an anti-inflammatory role in a variety of inflammatory diseases such as gastritis, hepatitis, and colitis. However, inflammation-regulatory activity the calyx P. berry has not been thoroughly evaluated. To understand whether portion is able to ameliorate processes, ethanolic extract (Pg-C-EE) was prepared, lipopolysaccharide-activated macrophages HEK293 cells transfected with proteins were used test action Pg-C-EE. The ginsenoside contents Pg-C-EE analyzed by HPLC. Suppressive on NO production, gene expression, transcriptional activation, inflammation signaling events examined using Griess assay, reverse transcription-polymerization chain reaction, luciferase reporter immunoblotting analysis. reduced production diminished mRNA expression genes cyclooxygenase-2, inducible synthase, tumor necrosis factor-α dose-dependent manner. This suppressed induced only nuclear factor-κB. Interestingly, analysis results demonstrated that activities protein kinase B (AKT)1 AKT2. These suggest may have nuclear-factor-κB-targeted properties through suppression AKT. underused part plant, development calyx-derived extracts be useful for treatment diseases.