The effects of Korean Red Ginseng-derived components on oligodendrocyte lineage cells: Distinct facilitatory roles of the non-saponin and saponin fractions, and Rb1, in proliferation, differentiation and myelination

• DOI: 10.1016/j.jgr.2021.04.007
• Publication Date: 2021-04-29T07:26:58Z
• Authors: Ahreum Lee; Oh Wook Kwon; Kwi Ryun Jung; Gyun Jee Song; Hyun-Jeong Yang
• Keywords: Gap-43 protein

Abnormalities of myelin, which increases the efficiency action potential conduction, are found in neurological disorders. Korean Red Ginseng (KRG) demonstrates therapeutic efficacy against some these conditions, however effects on oligodendrocyte (OL)s not well known. Here, we examined KRG-derived components development and protection OL-lineage cells.Primary OL precursor cell (OPC) cultures were prepared from neonatal mouse cortex. The protective efficacies KRG inhibitors mitochondrial respiratory chain activity. For vivo function Rb1 myelination, after 10 days oral gavage into adult male mice, forebrains collected. OPC proliferation assessed by BrdU incorporation, differentiation myelination qPCR, western blot immunocytochemistry.The non-saponin promoted proliferation, while saponin differentiation. Both processes mediated AKT extracellular regulated kinase (ERK) signaling. extract, protected OPCs oxidative stress, both extract significantly increased expression antioxidant enzyme. Among 11 major ginsenosides tested, membrane size vitro. Moreover, myelin formation brain.All prevented deaths under stress. While fraction size. Furthermore, among all tested ginsenosides, showed biggest increase enhanced vivo. These results imply potentials for myelin-related