Personalized peptide vaccine-induced immune response associated with long-term survival of a metastatic cholangiocarcinoma patient

• DOI: 10.1016/j.jhep.2016.06.027
• Publication Date: 2016-07-09T07:19:46Z
• Authors: Markus W. Löffler; P. Anoop Chandran; Karoline Laske; Christopher Schro...; Irina Bonzheim; Mathias Walzer; Franz J. Hilke; Nico Trautwein; Daniel J. Kowalewski; Heiko Schuster; Marc Günder; Viviana A. Carcam...; Christopher Mohr; Marc Sturm; Huu-Phuc Nguyen; Olaf Riess; Peter Bauer; Sven Nahnsen; Silvio Nadalin; Derek Zieker; Jörg Glatzle; Karolin Thiel; Nicole Schneiderh...; Stephan Clasen; Hans Bösmüller; Falko Fend; Oliver Kohlbacher; Cécile Gouttefangeas; Stefan Stevanović; Alfred Königsrainer; Hans-Georg Rammensee
• Keywords: Peptide vaccine; Cancer vaccine

We report a novel experimental immunotherapeutic approach in patient with metastatic intrahepatic cholangiocarcinoma. In the 5year course of disease, initial tumor mass, two local recurrences and lung metastasis were surgically removed. Lacking alternative treatment options, aiming at induction anti-tumor T cells responses, we initiated personalized multi-peptide vaccination, based on in-depth analysis antigens (immunopeptidome) sequencing.Tumors characterized by immunohistochemistry, next-generation sequencing mass spectrometry HLA ligands.Although several tumor-specific neo-epitopes predicted silico, none could be validated spectrometry. Instead, vaccine containing non-mutated tumor-associated epitopes was designed applied. Immunomonitoring showed vaccine-induced cell responses to three out seven peptides administered. The pulmonary resected after start vaccination strong immune infiltration perforin positivity, contrast previous lesions. remains clinically healthy, without any radiologically detectable tumors since March 2013 is continued.This remarkable clinical encourages formal studies adjuvant peptide cholangiocarcinoma.Metastatic cholangiocarcinomas, cancers that originate from liver bile ducts, have very limited options fatal prognosis. describe therapeutic such using vaccine. This vaccine, developed characterization patient's tumor, evoked associating long-term tumor-free survival.