Focal adhesion kinase (FAK) promotes cholangiocarcinoma development and progression via YAP activation

• DOI: 10.1016/j.jhep.2021.05.018
• Publication Date: 2021-05-28T04:51:38Z
• Authors: Xinhua Song; Hongwei Xu; Pan Wang; Jingxiao Wang; Silvia Affo; Haichuan Wang; Meng Xu; Binyong Liang; Li Che; Wei Qiu; Robert F. Schwabe; Tammy T. Chang; Marion Vogl; Giovanni M. Pes; Silvia Ribback; Matthias Evert; Xin Chen; Diego F. Calvisi
• Keywords: Liver Cancer; 570; Clinical Sciences; 610; Clinical sciences; California; Cholangiocarcinoma; Cohort Studies; Mice; Rare Diseases; intrahepatic cholangiocarcinoma; 2.1 Biological and endogenous factors; cancer; Animals; Digestive Diseases - (Gallbladder); Cancer; Biomedical and Clinical Sciences; FAK; Gastroenterology & Hepatology; Animal; Liver Disease; YAP-Signaling Proteins; cancer; FAK; intrahepatic cholangiocarcinoma; targeted therapy; YAP; Animals; California; Cholangiocarcinoma; Cohort Studies; Disease Models, Animal; Focal Adhesion Protein-Tyrosine Kinases; Mice; Signal Transduction; YAP-Signaling Proteins; targeted therapy; 3. Good health; Disease Models, Animal; 5.1 Pharmaceuticals; Focal Adhesion Protein-Tyrosine Kinases; Disease Models; Public Health and Health Services; YAP; Digestive Diseases; Signal Transduction

Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase that is upregulated in many tumor types and is a promising target for cancer therapy. Herein, we elucidated the functional role of FAK in intrahepatic cholangiocarcinoma (iCCA) development and progression.Expression levels and activation status of FAK were determined in human iCCA samples. The functional contribution of FAK to Akt/YAP murine iCCA initiation and progression was investigated using conditional Fak knockout mice and constitutive Cre or inducible Cre mice, respectively. The oncogenic potential of FAK was further examined via overexpression of FAK in mice. In vitro cell line studies and in vivo drug treatment were applied to address the therapeutic potential of targeting FAK for iCCA treatment.FAK was ubiquitously upregulated and activated in iCCA lesions. Ablation of FAK strongly delayed Akt/YAP-driven mouse iCCA initiation. FAK overexpression synergized with activated AKT to promote iCCA development and accelerated Akt/Jag1-driven cholangiocarcinogenesis. Mechanistically, FAK was required for YAP(Y357) phosphorylation, supporting the role of FAK as a central YAP regulator in iCCA. Significantly, ablation of FAK after Akt/YAP-dependent iCCA formation strongly suppressed tumor progression in mice. Furthermore, a remarkable iCCA growth reduction was achieved when a FAK inhibitor and palbociclib, a CDK4/6 inhibitor, were administered simultaneously in human iCCA cell lines and Akt/YAP mice.FAK activation contributes to the initiation and progression of iCCA by inducing the YAP proto-oncogene. Targeting FAK, either alone or in combination with anti-CDK4/6 inhibitors, may be an effective strategy for iCCA treatment.We found that the protein FAK (focal adhesion kinase) is upregulated and activated in human and mouse intrahepatic cholangiocarcinoma samples. FAK promotes intrahepatic cholangiocarcinoma development, whereas deletion of FAK strongly suppresses its initiation and progression. Combined FAK and CDK4/6 inhibitor treatment had a strong anti-cancer effect in in vitro and in vivo models. This combination therapy might represent a valuable and novel treatment against human intrahepatic cholangiocarcinoma.