Genome-wide analysis of AhCN genes reveals the AhCN34 involved in bacterial wilt resistance in peanut

• DOI: 10.1016/j.jia.2024.03.006
• Publication Date: 2024-03-02T02:27:29Z
• Authors: Kai Zhao; Yanzhe Li; Zhan Li; Zenghui Cao; Xingli Ma; Rui Ren; Kuopeng Wang; Lin Meng; Yang Yang; Miaomiao Yao; Yang Yang; Xiaoxuan Wang; Jinzhi Wang; Sasa Hu; Yaoyao Li; Qian Ma; Di Cao; Kunkun Zhao; Ding Qiu; Fangping Gong; Zhongfeng Li; Xingguo Zhang; Dongmei Yin
• Keywords: Bacterial wilt

Peanut (Arachis hypogaea L.) bacterial wilt (BW), caused by Ralstonia solanacearum (RS), is a devastating soil-borne disease that poses significant threat to peanut yield and quality. Nucleotide-binding leucine-rich repeat (NBS-LRR) proteins are class of plant-specific immune receptors recognize pathogen-secreted effector molecules activate responses resist pathogen infections. However, the precise functions AhCN genes (CN NLR lacking LRR structural domains) in plants not fully understood. In this study, total 150 were identified classified into nine subfamilies based on systematic phylogenetic analysis. The showed highly conserved features; promoter cis-elements indicated involvement plant hormone signaling defense responses. Following inoculation with RS, resistant variety 'H108' significantly outperformed susceptible 'H107' physiological indicators such as height, main stem diameter, fresh weight, likely due inhibition proliferation diffusion vascular bundle. AhCN34 was found be upregulated H108 compared H107 during infection response treatment each three hormones. Importantly, overexpression leaves enhanced their resistance BW. These findings demonstrate great potential for applications breeding. Our identification characterization provide insights mechanisms underlying BW inform future research genetic methods improving resistance.