miRNA-203b-3p Induces Acute and Chronic Pruritus through 5-HTR2B and TRPV4

• DOI: 10.1016/j.jid.2022.08.034
• Publication Date: 2022-08-30T01:01:29Z
• Authors: Francesco De Logu; Roberto Maglie; Mustafa Titiz; Giulio Poli; Lorenzo Landini; Matilde Marini; Daniel Souza Mont...; Gaetano De Siena; Marco Montini; Daniela Almeida C...; Michel Fleith Otuki; Priscila Lúcia Pa...; Emiliano Antiga; Tiziano Tuccinardi; João Batista Calixto; Pierangelo Geppetti; Romina Nassini; Eunice André
• Keywords: TRPV4

Growing evidence indicates that transient receptor potential (TRP) channels contribute to different forms of pruritus. However, the endogenous mediators cause itch through signaling are poorly understood. In this study, we show genetic deletion or pharmacological antagonism TRPV4 attenuated in a mouse model psoriasis induced by topical application imiquimod. Human psoriatic lesions showed increased expression several microRNAs, including miR-203b-3p, which calcium ion response rodent dorsal root ganglion neurons and scratching behavior mice 5-HTR2B activation protein kinase C‒dependent phosphorylation TRPV4. Computer simulation revealed miR-203b-3p core sequence (GUUAAGAA) causes 5-HTR2B/TRPV4-dependent targets extracellular side interacting with portion pocket consistent its activation. Overall, reveal unconventional pathophysiological role an microRNA can behave as promoter