Low cost, disposable biosensors allow detection of antibodies with results equivalent to ELISA in 15min

• DOI: 10.1016/j.jim.2012.10.007
• Publication Date: 2012-10-23T00:01:53Z
• Authors: Jennifer Cork; Rebecca M. Jones; Jason Sawyer
• Keywords: Time Factors; Cost-Benefit Analysis; Reproducibility of Results; Enzyme-Linked Immunosorbent Assay; Biosensing Techniques; 02 engineering and technology; Antibodies, Viral; Sensitivity and Specificity; Milk; Animals; Cattle; 0210 nano-technology; Infectious Bovine Rhinotracheitis; Herpesvirus 1, Bovine

An assay for detection of antibodies to bovine herpesvirus-1 (BoHV-1) was developed by combining a commercial low cost, disposable biosensor system (Vantix™) and reagents from an established Enzyme-Linked Immunosorbent Assay (ELISA). The biosensor assay produced equivalent results to ELISA within 15 min when testing 194 bovine serum and 50 bulk milk samples submitted for routine testing. The biosensor assay can provide quantitative analysis demonstrated by measuring the level of antibody in milk samples. The results of this study suggest that Vantix™ is a promising platform for routine immunological testing. The technology may be particularly useful for low to medium throughput tests where rapid results are required. The biosensors could also form the basis of a future point-of-care test platform.