Methanolic extract of coconut (Cocos nucifera L.) haustorium mitigates pro-oxidant-mediated apoptotic cell death via Nrf-2 pathway and lipopolysaccharide-induced cytokine release in cells

• DOI: 10.1016/j.jksus.2021.101715
• Publication Date: 2021-11-16T17:21:55Z
• Authors: Hak-Jae Kim; Arunaksharan Nara...; Anju Sasidharan; Joice Tom Job; Young Ock Kim; Sae Won Na; Rajakrishnan Raja...; Ahmed Alfarhan; Bander Al-Munqedhi
• Keywords: Malondialdehyde; Glutathione reductase

Cocos nucifera L is well-known for its edible and medicinal uses; multiple products from the different parts of plant are being used by various communities in India. A less explored product coconut haustorium, solid endosperm portion, which highly nutritious. The present study first time evaluated antioxidant, anti-inflammatory cytoprotective properties methanolic extract Coconut haustorium (CHE). effect CHE was hydrogen peroxide/malondialdehyde-induced toxicity IEC-6 (normal intestinal cell). Initially biologically safe concentration IC50 value peroxide malondialdehyde estimated MTT assay. Treatment with H2O2 MDA led to glutathione depletion reduction catalase reductase activity; resulted increased oxidative stress thereby cell death. Pretreatment concentrations (10, 20 40 µg/mL) offered significant dose-dependent protection against induced malondialdehyde. It observed that pretreatment restored antioxidant enzyme activities GSH levels back normal; improvement status may be through upregulation Nrf2/HO1 pathway as pretreated cells. potential lipopolysaccharide (LPS-1 model macrophages (Raw 264.7 cells). reduced LPS- release cytokines such IL-1β, IL-6, tumor necrosis factor-alpha (TNF-α). results indicated Coconut.