Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish

• DOI: 10.1016/j.jlr.2021.100142
• Publication Date: 2021-10-18T15:19:43Z
• Authors: Megan M. Knuth; Whitney L. Stutts; Morgan M. Ritter; Kenneth P. Garrard; Seth W. Kullman
• Keywords: 2-Arachidonoylglycerol

Vitamin D is well known for its traditional role in bone mineral homeostasis; however, recent evidence suggests that vitamin also plays a significant metabolic control. This study served to investigate putative linkages between deficiency (VDD) and disruption of bioactive lipids by MS imaging. Our approach employed infrared-matrix-assisted laser desorption electrospray ionization imaging lipid metabolite profiling 6-month-old zebrafish fed either VDD or D-sufficient (VDS) diet. Using lipidomics pipeline, we found had greater abundance (N-acyls, endocannabinoids [ECs], diacylglycerols/triacylglycerols, bile acids/bile alcohols, derivatives) suggestive increased EC tone compared with VDS zebrafish. Tandem was performed on several differentially expressed metabolites sufficient ion abundances aid structural elucidation provide additional support annotations. To confirm activation the pathways, subsequently examined expression genes involved biosynthesis, metabolism, receptor signaling adipose tissue liver from Gene changes were congruent tone, demonstrating synthesis metabolism anandamide Taken together, our data suggest may promote accumulation