Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma

• DOI: 10.1016/j.jlr.2021.100144
• Publication Date: 2021-10-26T22:19:38Z
• Authors: Oleg Kovrov; Fredrik Landfors; Valeria Saar-Kovrov; Ulf Näslund; Gunilla Olivecrona
• Keywords: Isothermal Titration Calorimetry

LPL is a key player in plasma triglyceride metabolism. Consequently, regulated by several proteins during synthesis, folding, secretion, and transport to its site of action at the luminal side capillaries, as well catalytic reaction. Some are known, whereas others have been identified but still not fully understood. We set out study effects natural variations levels all known regulators on activity purified added samples fasted taken from 117 individuals. The enzymatic was measured 25°C using isothermal titration calorimetry. This method allows quantification ability an fixed amount exogenous hydrolyze triglyceride-rich lipoproteins measuring heat produced. Our results indicate that, under conditions used, normal variation endogenous apolipoprotein C1, C2, C3 or angiopoietin-like 3, 4, 8 had no significant effect recorded LPL. Instead, determinant for lipid signature strongly correlated average size VLDL particles. involved only lipoprotein parameters also A5 levels. While measurements cannot represent when attached capillary wall, our provides knowledge interindividual lipolysis rates human plasma.