The Urinary Bladder is Rich in Glycosphingolipids Composed of Phytoceramides

• DOI: 10.1016/j.jlr.2022.100303
• Publication Date: 2022-10-27T06:11:12Z
• Authors: Takashi Watanabe; Akemi Suzuki; Shin Ohira; Shinji Go; Yuta Ishizuka; Takuya Moriya; Yoshiyuki Miyaji; Tota Nakatsuka; Keita Hirata; Atsushi Nagai; Junko Matsuda
• Keywords: Globotriaosylceramide; Lactosylceramide; Sphingolipid; Glycosphingolipid

Glycosphingolipids (GSLs) are composed of a polar glycan chain and hydrophobic tail known as ceramide. Together with variation in the chain, ceramides exhibit tissue-specific structural long-chain base (LCB) N-acyl moieties terms carbon length, degree desaturation, hydroxylation. Here, we report GSLs urinary bladders mice humans. Using TLC, showed that major hexosylceramide, lactosylceramide, globotriaosylceramide, globotetraosylceramide, Neu5Ac-Gal-Glc-Ceramide, Neu5Ac-Neu5Ac-Gal-Glc-Ceramide. Our LC-MS analysis indicated phytoceramide structures 20-carbon LCB (4-hydroxyeicosasphinganine) 2-hydroxy fatty acids abundant hexosylceramide Neu5Ac-Gal-Glc-Ceramide In addition, quantitative PCR demonstrated DES2 FA2H, which responsible for generation 4-hydroxysphinganine acid, respectively, SPTLC3 SPTSSB, LCBs, significant expressions epithelial layer than subepithelial layer. Immunohistochemically, dihydroceramide:sphinganine C4-hydroxylase (DES2) was expressed exclusively urothelial cells bladder. findings suggest these ceramide have an impact on membrane properties stretching shrinking transitional cells.