Separation of extra- and intracellular metabolites using hyperpolarized 13C diffusion weighted MR
Pyruvate
Magnetic Resonance Spectroscopy
Biophysics
610
Diffusion weighted magnetic resonance
Cell Line
03 medical and health sciences
Engineering
Bioreactors
0302 clinical medicine
Dynamic nuclear polarization
Cell Line, Tumor
616
Hyperpolarized (13)C magnetic resonance (HP (13)C MR)
Pyruvic Acid
Hyperpolarized C-13 magnetic resonance
Cellular transport
Humans
Lactate efflux
Lactic Acid
Cancer
Carbon Isotopes
Tumor
Magnetic Resonance Imaging
Renal cell carcinoma
Physical sciences
Physical Sciences
Cancer aggressiveness
Biomedical Imaging
Lactate
Aerobic glycolysis
DOI:
10.1016/j.jmr.2016.07.002
Publication Date:
2016-07-10T05:02:39Z
AUTHORS (10)
ABSTRACT
This work demonstrates the separation of extra- and intracellular components of glycolytic metabolites with diffusion weighted hyperpolarized (13)C magnetic resonance spectroscopy. Using b-values of up to 15,000smm(-2), a multi-exponential signal response was measured for hyperpolarized [1-(13)C] pyruvate and lactate. By fitting the fast and slow asymptotes of these curves, their extra- and intracellular weighted diffusion coefficients were determined in cells perfused in a MR compatible bioreactor. In addition to measuring intracellular weighted diffusion, extra- and intracellular weighted hyperpolarized (13)C metabolites pools are assessed in real-time, including their modulation with inhibition of monocarboxylate transporters. These studies demonstrate the ability to simultaneously assess membrane transport in addition to enzymatic activity with the use of diffusion weighted hyperpolarized (13)C MR. This technique could be an indispensible tool to evaluate the impact of microenvironment on the presence, aggressiveness and metastatic potential of a variety of cancers.
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CITATIONS (18)
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