Cooked common beans ( Phaseolus vulgaris L.) modulate renal genes in streptozotocin-induced diabetic rats

Blood Glucose Male Phaseolus 0301 basic medicine 2. Zero hunger Gene Expression Profiling Kidney Streptozocin Diabetes Mellitus, Experimental 03 medical and health sciences Gene Expression Regulation Functional Food Creatinine Hyperglycemia Seeds Albuminuria Animals Urea Diabetic Nephropathies Cooking Rats, Wistar
DOI: 10.1016/j.jnutbio.2015.02.006 Publication Date: 2015-04-11T04:30:32Z
ABSTRACT
Food consumption with different bioactive compounds could reduce the risk of diabetic complications. This study was designed to evaluate the effect of cooked common beans on differentially expressed genes in whole kidney homogenates of streptozotocin-induced diabetic rats. After 4weeks of treatment with a cooked bean supplemented (10%) diet, animals fed with Flor de Mayo bean (FMB) exerted the greatest protective effect, since they presented the lowest blood glucose levels, consistent with an increase in blood insulin levels, a decrease in urine albumin and urea levels and an increase in creatinine clearance (P≤.05). Regarding the gene expression of kidneys evaluated using expressed sequence tag, consumption of cooked beans improved the expression of Glu1, Cps1, Ipmk, Cacna1c, Camk1, Pdhb, Ptbp3 and Pim1, which are related to the elimination of ammonium groups, the regulation of inflammatory and oxidative response, as well as cell signaling and apoptosis. In addition, the beneficial effects observed were not related to their polyphenolic and saponin profile, suggesting the activity of other bioactive compounds or the synergistic interaction of these compounds. These results suggest that the consumption of cooked common beans (FMB) might be used as an alternative for the regulation of genes related to renal alterations.
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