Unilateral cervical spinal cord injury induces bone loss and metabolic changes in non-human primates (Macaca fascicularis)

• DOI: 10.1016/j.jot.2021.03.006
• Publication Date: 2021-06-07T17:47:39Z
• Authors: Xiuhua Wu; Xiaolin Xu; Qi Liu; Jianyang Ding; Junhao Liu; Zhiping Huang; Zucheng Huang; Xiaoliang Wu; Rong Li; Zhou Yang; Hui Jiang; Jie Liu; Qingan Zhu
• Keywords: 03 medical and health sciences; 0302 clinical medicine; RC925-935; Bone microarchitecture; Osteoporosis; Non-human primates (NHP); Original Article; Spinal cord injury; Diseases of the musculoskeletal system; 3. Good health

The deleterious effects of chronic spinal cord injury (SCI) on the skeleton in rats, especially lower extremities, has been proved previously. However, long-term skeletal changes after SCI non-human primates (NHP) have scarcely studied. This study aimed to evaluate bone loss limbs and vertebrae metabolic NHP unilateral cervical contusion injury. Twelve Macaca fascicularis were randomly divided into (n=8) Sham (n=4) groups. models established using hemi-contusion fifth vertebra (C5), further evaluated by histological staining neurophysiological monitoring. Changes microstructures, biomechanics, metabolism markers assessed micro-CT, micro-FEA serological kit. model led consistent limb dysfunction potential plasticity face cord. Furthermore, cancellous mass ipsilateral humerus radius decreased significantly compared contralateral side. volume fraction 17.2% 20.1% while 29.0% 30.1% respectively. Similarly, thickness cortical forelimbs was decreased, as well strength forelimbs. These accompanied diminished concentration osteocalcin total procollagen type 1 N-terminal propeptide (t-P1NP) increased level β-C-terminal cross-linking telopeptide 1collagen (β-CTX) testing. present demonstrated that hemi-SCI induced compromised biomechanical performance forelimbs, which could be indicated both muscle atrophy metabolism, associated with a large-diameter cells sensory neurons dorsal root ganglia. Our study, for first time, (SCI). may help elucidate role atrophy, mechanisms SCI-induced osteoporosis, would definitely better rodent models.