Controlled growth factor delivery system with osteogenic-angiogenic coupling effect for bone regeneration

• DOI: 10.1016/j.jot.2021.11.004
• Publication Date: 2021-12-11T10:09:56Z
• Authors: Fei Kang; Qiying Yi; Pengcheng Gu; Yuhan Dong; Ziyang Zhang; Lijuan Zhang; Yan Bai
• Keywords: Delivery system

Bone regeneration involves a coordinated cascade of events that are regulated by several cytokines and growth factors, among which bone morphogenic protein-2 (BMP-2), vascular endothelial factor (VEGF) fibroblast factor-2 (FGF-2) play important roles. In this study, we investigated the effects dual release three factors on in femur defects.A composite consisting Gelatin microparticles loaded with VEGF/FGF-2 poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) BMP-2 encapsulated nano hydroxyapatite-poly actic-co-glycolic acid (nHA-PLGA) scaffold was prepared for factors.On 14th day, decreased rate compared FGF-2 VEGF observed. However, after 14 days, to VEGF, showed an increased rate. Controlled resulted significant osteogenic differentiation mesenchymal stem cells (BMSCs). Moreover, functional connection osteoblast-vascular during development were evaluated. The synergistic delivery shown promote expression BMSCs. Increased secretion from BMSCs promoted proliferation angiogenic human umbilical vein (HUVECs) co-culture system. At 12 weeks implantation, blood vessel formation analyzed micro-CT histology. significantly vessels new defects.These findings demonstrate PLGA-PEG-COOH microparticles-based scaffolds exerted osteogenic-angiogenic coupling effect regeneration. This approach will inform appropriate designs high-performance bioscaffolds tissue engineering.