l-arginine promotes angio-osteogenesis to enhance oxidative stress-inhibited bone formation by ameliorating mitophagy

• DOI: 10.1016/j.jot.2024.03.003
• Publication Date: 2024-05-21T03:15:26Z
• Authors: Yang Shen; Haoming Wang; Hongwei Xie; Jiateng Zhang; Qingliang Ma; Shiyu Wang; Putao Yuan; Hong Xue; Huaxing Hong; Shunwu Fan; Wenbin Xu; Ziang Xie
• Keywords: angiogenesis; 0303 health sciences; 03 medical and health sciences; mitophagy; RC925-935; Osteogenesis; Original Article; l-arginine; Diseases of the musculoskeletal system

Osteoporosis is one of the most common bone diseases in middle-aged and elderly populations worldwide. The development new drugs to treat disease a key focus research. Current treatments for osteoporosis are mainly directed at promoting osteoblasts inhibiting osteoclasts. However, there currently no ideal approach treatment. l-arginine semi-essential amino acid involved number cellular processes, including nitric production, protein biosynthesis, immune responses. We previously reported that l-arginine-derived compounds can play regulatory role homeostasis. To investigate specific effect on Mildly aged ovariectomized mouse models were used study effects osteogenesis angiogenesis, assessed by micro-computed tomography immunostaining tissue. osteogenesis, adipogenesis was further studied vitro using obtained from cranial cap bone, endothelial cells, an adipogenic cell line. Specific methods assess these processes included lipid staining, migration, tube-forming, wound-healing assays. Protein mRNA expression determined select biomarkers. found attenuated loss promoted angiogenesis. increased activity vascular whereas it inhibited vitro. In addition, we altered PINK1/Parkin Bnip3 mitochondria osteoblast-lineage thereby mitophagy protecting cells ROS. Similarly, treatment effectively ameliorated model. promotes angio-osteogenesis, inhibits adipogenesis, mediated PINK1/Parkin- Bnip3-mediated mitophagy. L-arginine supplementation may be effective adjunct therapy osteoporosis.