Multifaceted roles of the egg perivitelline layer in avian reproduction: Functional insights from the proteomes of chicken egg inner and outer sublayers

• DOI: 10.1016/j.jprot.2022.104489
• Publication Date: 2022-01-20T17:05:06Z
• Authors: Mégane Brégeon; Daniel Tomas; Benoît Bernay; Céline Zatylny-Ga...; Sonia Georgeault; Valérie Labas; Sophie Réhault-Go...; Nicolas Guyot
• Keywords: Perivitelline space

The avian egg perivitelline layer (PL) is a proteinaceous structure that encloses the yolk. It consists of inner and outer layers (IPL OPL, respectively) are assumed to play distinct roles in bird reproduction. To gain insight into their respective function, we analyzed proteome IPL OPL chicken unfertilized eggs after mechanical separation, using GeLC-MS/MS strategy. Of 412 proteins identified, 173 were uniquely recovered 98 while 141 identified both sublayers. Genes coding most abundant shown be expressed either liver/ovary formation) or oviduct (OPL formation), but rarely both. presence oviduct-specific (including LYZ, VMO1, AvBD11, PTN, OVAL LOC10175704) strongly suggests they participate physical association whose tight attachment was further evidenced by analyses IPL/OPL interfaces (by scanning electron microscopy). Functional annotation revealed functions associated with fertilization early development for IPL, would rather defense embryogenesis. Collectively, our data highlight complementary major determinants reproductive success. present study unveils first time individual proteomes two sublayers composing (PL), which allowed assign putative biological combination proteomics gene expression ultrastructural provides insightful on biochemistry PL. functional PL highlights multifaceted this reproduction including fertilization, embryonic development, antimicrobial protection. This work will stimulate research validate predicted compare physiology specificities egg-laying species.