Extraction of malva sylvestris seed oil by soxhlet method and alginate/chitosan microencapsulation with a new method: Investigation of its cytotoxic effects on human neuroblastoma cell line (SH-SY5Y)

• DOI: 10.1016/j.jrras.2024.100875
• Publication Date: 2024-03-06T13:04:47Z
• Authors: Hasan Ulusal; Fatma Ulusal; Sibel Dagli; Cahit Toprak
• Keywords: SH-SY5Y

Biopolymers such as chitosan or alginate are at the forefront in healthcare applications due to their broad bioactivities. Biomolecules obtained by loading plant extracts into capsules prepared with and combinations show anticancer, antioxidant antimicrobial properties. In this study, Malva sylvestris seed oil (MSSO) was extracted Soxhlet extraction method alginate/chitosan encapsulation performed a new method. The anticancer properties of were examined human neuroblastoma cell line (SH-SY5Y). For purpose, MSSO separated maceration (Extract 1) soxhelet 2) microencapsulated (MicroCapsule 1 MicroCapsule 2). Encapsulation efficiency (EE%) drug (DL%) calculated for each two capsules. cytotoxic effects microcapsules CCK-8 cytotoxicity test on SH-SY5Y 24 48 h incubation periods. EE DL microcapsule 94.10% 4.92%, respectively, 2 86.69% 3.15%, respectively. IC50 values using Graphad Prism 10.0.2. As result extracts, it found that both had approximately 5 times lower values. Additionally, slow release from Since microencapsulation process increases amount substance unit volume provides controlled drug, doses. This will allow be more concentrated cancerous cells tissues overcome resistance may develop cell.