Construction of an aptasensor based on the composite nanomaterial IL-rGO-TEPA/SiO2-AuPt and its high-sensitive detection of cardiac troponin I

• DOI: 10.1016/j.jscs.2024.101898
• Publication Date: 2024-06-27T18:46:39Z
• Authors: Jiaxin Wang; Jian Mao; Qinghua Yan; Li Yang
• Keywords: Chemistry; Composite nanomaterials IL-rGO-TEPA/SiO2-AuPt; Cardiac troponin I; Aptasensor; 02 engineering and technology; 0210 nano-technology; QD1-999

To accurately diagnose and evaluate the degree of myocardial injury predict infarction disease, rapid sensitive detection cardiac troponin I (cTnI) is required. Therefore, a label-less electrochemical aptasensor was prepared for cTnI determination. Ionic liquid 1-hydroxyethy l-3- methyl imidazole four fluorine boric acid salt (IL)-reduced graphene oxide − ethylene oxidation reduction five amine (rGO-TEPA)/silica (SiO2)- gold platinum (AuPt) (IL-rGO-TEPA/SiO2-AuPt) nanocomposites were synthetized as substrate nanocomposite characterized using series characterization methods. The performance through Result displayed that better materials synthesized, showed excellent performance. Under optimal conditions, had salient analytical signals linearly decreased with logarithm concentration between 0.5 pg·mL−1 1 × 106 range (R2 value = 0.9955) wide (0.5 ∼ pg·mL−1) low limit 0.4 pg·mL−1. At same time, good selectivity, repeatability stability. Relative standard deviation (RSD) values ranged from 3.45 % 4.97 recovery rate 97.5 103 in human serum samples, RSD spiked ELISA method less than 5 %. This study provides new theoretical basis clinical cTnI.