Topical fluorometholone treatment and desiccating stress change inflammatory protein expression in tears

• DOI: 10.1016/j.jtos.2017.09.003
• Publication Date: 2017-09-14T07:50:48Z
• Authors: Janika Nättinen; Antti Jylhä; Ulla Aapola; Amalia Enríquez-d...; José Pinto-Fraga; Alberto López-Miguel; María J. González...; Michael E. Stern; Margarita Calonge; Lei Zhou; Matti Nykter; Hannu Uusitalo; Roger Beuerman
• Keywords: Expression (computer science)

It was hypothesized that tear protein biomarkers could predict the effects of topical steroid treatment and desiccating stress in patients with dry eye disease (DED). To test this concept, a randomized, double-masked, controlled clinical trial 41 conducted. The were treated topically either 0.1% fluorometholone (FML) or polyvinyl alcohol (PA). Tear samples collected using 1 μl glass capillaries at recruitment into study after 3-week period, both before 2 h exposure to stress, environment chamber. Relative quantification proteins conducted by NanoLC-MSTOF sequential window acquisition all theoretical mass spectra (SWATH). Ocular surface integrity (corneal conjunctival staining hyperemia) selected as key DED-related sign analyzed proteomic data. Analysis covariance (ANCOVA) linear models used analyze data R. 758 identified relatively quantified from each sample. revealed 9 differentially expressed between FML PA treatments 3 weeks 7 (P < 0.05). We also several initial collection, which be changes corneal hyperemia stress. These include complement C3 (C3) calmodulin like 5 (CALML5), differentiate severity DED baseline. further identify most benefiting treatment.