Antioxidant, a pro-oxidant and cytotoxic effects of Achyrocline satureioides extracts

• DOI: 10.1016/j.lfs.2003.09.073
• Publication Date: 2004-03-14T10:07:16Z
• Authors: M Polydoro; K.C.B de Souza; M.E Andrades; E.G Da Silva; F Bonatto; J Heydrich; F Dal-Pizzol; E.E.S Schapoval; V.L Bassani; J.C.F Moreira
• Keywords: Flavonoids; Male; 0301 basic medicine; Achyrocline; Plants, Medicinal; Sertoli Cells; Dose-Response Relationship, Drug; Cell Survival; Plant Extracts; Thiobarbituric Acid Reactive Substances; Antioxidants; Rats; 3. Good health; 03 medical and health sciences; Animals; Lipid Peroxidation; Rats, Wistar; Reactive Oxygen Species; Brazil; Cells, Cultured; Chromatography, High Pressure Liquid

In this study we compared the antioxidant properties of five different extracts of different composition obtained from Achyrocline satureioides' inflorescences (Compositae), a widely used Brazilian folk medicinal herb. All of the extracts presented significant antioxidant potential identified by TRAP assay, which increased in the presence of human plasma. Characterization of the content of flavonoids in each extract showed that the FDP80 (ethanol 80%) and FFr (enriched flavonoid fraction) extracts contained a higher content of flavonoids. Cytotoxicity of the extracts as determined in Sertoli cell culture showed that FDP80 and FFr were highly toxic at most concentrations tested. The extracts induced a significant increase in lipid peroxidation levels in Sertoli cells. These results suggest that medicinal herb extracts that contain higher flavonoid concentrations and shows higher antioxidant protection in vitro might not always produce the greatest benefit.