Hyperglycemic levels in early stage of diabetic nephropathy affect differentially renal expression of claudins-2 and -5 by oxidative stress

• DOI: 10.1016/j.lfs.2020.119003
• Publication Date: 2021-01-07T19:52:18Z
• Authors: Lorena Rosas-Mart...; Rafael Rodríguez-...; María del Carmen ...; Fanis Missirlis; Leonardo del Vall...; Alicia Sánchez-Me...; José L. Reyes-Sán...; Luz Graciela Cerv...
• Keywords: Malondialdehyde

This study attempts to elicit whether the level of hyperglycemia in an early stage diabetic nephropathy changes renal expression claudins-2 and -5 determine involvement glucose-induced oxidative stress. Streptozotocin-induced type-1 type-2 (DM1, DM2)-rat models were used. At 14-week old, rats placed metabolic cages evaluate proteinuria, creatinine clearance, electrolyte excretion. Proximal tubules glomeruli isolated analyzed by Western blot immunofluorescence. Renal stress metalloproteinase activities evaluated. We found that claudin-5 claudin-2 proximal significantly reduced DM1 versus DM2 model, paralleling with higher proteinuria loss sodium potassium reabsorption, increased malondialdehyde levels, but lower antioxidant capacity both models. Enzymatic activity MMP-2 and-9 was groups control being than DM2, suggesting claudin's degradation. The determines time-dependent progression nephropathy; hyperglycemia-induced parallels increase metalloproteinases (MMPs) consequently affecting integrity glomerulus tubule. Our results suggest chronic high-glycemia levels stages decrease -5, stress, induce MMP-activity faster middle-glycemia levels.