Ingestion of mannose ameliorates thioacetamide-induced intrahepatic oxidative stress, inflammation and fibrosis in rats

• DOI: 10.1016/j.lfs.2021.120040
• Publication Date: 2021-10-11T14:57:32Z
• Authors: Mohamed E. Shaker; Nada H. Eisa; Abdelaziz Elgaml; Ahmed El-Mesery; Mohamed El-Shafey; Mohamed El-Dosoky; Mohammed El-Mowafy; Mohamed El-Mesery
• Keywords: Inflammation; Liver Cirrhosis; Male; 0303 health sciences; Thioacetamide; Rats; 3. Good health; Rats, Sprague-Dawley; Oxidative Stress; 03 medical and health sciences; Liver; Malondialdehyde; Hepatic Stellate Cells; Animals; Cytokines; Mannose; Signal Transduction

The monosaccharide mannose has gained recent interest for its beneficial effect against certain inflammatory disorders. Nevertheless, the influence of mannose on experimentally-induced liver fibrosis and the ensued inflammation is still not fully clear to date.The current study investigated the outcomes of treating rats with mannose (0.2 ml of 20% w/v, oral gavage) 30 min before the twice weekly intoxication with thioacetamide (TAA) (200 mg/kg, intraperitoneal) for a total period of 8 weeks.The data indicated that mannose markedly dampened TAA-induced liver fibrosis, as indicated by lowering the fibrotic bridges shown by Masson's trichrome staining. This effect was consistent with reducing TAA-induced hepatocellular injury, as evidenced biochemically (serum ALT and AST activities) and pathologically (necroinflammation score). These hepatoprotective effects mediated by mannose were attributed to i) reversing TAA-induced rise in malondialdehyde (MDA) and decrease in reduced glutathione (GSH) expressions in the liver, ii) limiting TAA-induced release of the proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), iii) impairing TAA-induced activation of hepatic stellate cells by downregulating α-smooth muscle actin expression (α-SMA), and more importantly, iv) dampening TAA-induced fibrogenesis driven by transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF).Mannose may be a valuable candidate for preventing oxidative stress, inflammation and fibrogenesis in the liver.